22) In an analysis of the nucleotide composition of DNA, which of the following

Question

22) In an analysis of the nucleotide composition of DNA, which of the following will be found? A)A-c C)A+C-G+T D)G+C-T+A 23) An Okazaki fragment has which of the following arrangements? A) primase, polymerase, ligase B) 3' RNA nucleotides, DNA nucleotides 5 C) 5' RNA nucleotides, DNA nucleotides 3' D) DNA polymerase I, DNA polymerase I1 24) Which enzyme catalyzes the elongation of a DNA strand in the 53 direction? A) primase B) DNA ligase C) DNA polymerase II1 D) topoisomerase 25) In which order do the necessary enzymes act to repair a thymine dimer by nucleotide excision repair? A) nuclease, DNA polymerase, RNA primase B) helicase, DNA polymerase, DNA ligase C) DNA ligase, nuclease, helicase D) nuclease, DNA polymerase, DNA ligase 26) What is the function of DNA polymerase III? A) to unwind the DNA helix during replication B) to seal together the broken ends of DNA strands C) to add nucleotides to the 3' end of a growing DNA strand D) to degrade damaged DNA molecules 27) The leading and the lagging strands of DNA formed during DNA replication differ in that A) the leading strand is synthesized in the same direction as the movement of the replication fork, and the lagging strand is synthesized in the opposite direction. B) the leading strand is synthesized by adding nucleotides to the 3' end of the growing strand, and the lagging strand is synthesized by adding nucleotides to the 5' end. C) the lagging strand is synthesized continuously, whereas the leading strand is synthesized in short fragments that are ultimately stitched together D) the leading strand is synthesized at twice the rate of the lagging strand. 28) A new DNA strand elongates only in the 5 to 3' direction because A) DNA polymerase begins adding nucleotides at the 5' end of the template. B) Okazaki fragments prevent elongation in the 3' to 5' direction. C) replication must progress toward the replication fork. D) DNA polymerase can only add nucleotides to the free 3' end.

Explanation / Answer

22)c)A+C=G+T

Adenine binds with thymine

Guanine binds with cytosine

23)c) 5' RNA nucleotide, DNA nucleotide 3'

These are short newly synthesized DNA fragment

Formed during DNA replication

24)c) DNA polymerase lll

It is a holoenzyme

Involved in prokaryotic DNA replication

25)d) nuclease,DNA polymerase, ligase

Nuclease cleaves the chain of nucleotide

Polymerase synthesizes long chain of DNA molecules

Ligase helps in joining two large molecules

26)c) to add nucleotides to the end of the growing DNA strand

It is a holoenzyme

It involves in replication process

27)a) leading strand is synthesized in the same direction as the movement of replication fork

Lagging strand is synthesized in the opposite direction

28)d) DNA polymerase can only add nucleotides to the free 3' end

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