22 Transduction: 1. What is the purpose of a generalized transduction? (1 pt) 2.

Question

22 Transduction: 1. What is the purpose of a generalized transduction? (1 pt) 2. What mistake made during the P22 lytie cycle are resecarchers able to capitalize on to perform a transduction? (1 pt) What process occurs during transduction to allow packaged DNA from the P22 (phage) to integrate into the host chromosome? (1 pt) 3. 4. Name the 4 steps of the lytic cycle, including a brief description of each. (2 pts) You are given three different strains of S. enterica for a transduction experiment. Strain I has a wild-type phenotype, strain 2 has a defect in oligosaccharide synthesis and strain 3 is defective in Histidine synthesis? Which of these strains make a suitable host for successful transduction of the cam* (chloramphenicol resistance) gene using P22? Give reasons for your answer. (2 pts) 5.

Explanation / Answer

1- In generalised transduction one gene from a bacterium is transfered to other via a bacteriophage.

2) 0.3% of the time ,p1 mistakenly packages chromosomal DNA instead of its own.

3) homologus recombination - method in which nucleotide sequences are exchanged between two identical molecules of DNA.

4) Steps in the lytic cycle:

Related Questions

Navigate

• Browse (All)
• Browse 2
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.