5) In a paper from 1972 Plageman described an experiment where he fed yeast a si
ID: 219898 • Letter: 5
Question
5) In a paper from 1972 Plageman described an experiment where he fed yeast a single radioactive nucleoside. The cells took up these nucleosides and added them to their pool of RNA nucleotides. When he looked at which nucleotides within the RNA that was produced in these cells was radioactive he found the following results. (the - means that no appreciable levels of radioactivity were found in that nucleotide) Radioactive nucleoside Percentage of total radioactivity found in the RNA of the yeast CMP AMP UMP 4790 5% ??? GMP 53% 95% Uridine Cytidine Guanosine Inosine Adenosine 7% 51% 65% 93% 49% 35% Given what we talked about in class, explain the results above.Explanation / Answer
1) uridine is converted into either cytosine or uracil nucleotide equally. after formation of uridine, it converts into UTP and with the reaction of CTP synthetase UTP is converted into CTP. so approx equal % of CTP and UTP will radioactive.
2) after converting cytidine it will produce radioactive CTP.
3) purine synthesis IMP is either converted into Guanosine or Adenosine.
Guanosine converted into GTP and Adenosine will convert into ATP.
from Guanosine it converted into GTP maximum. and few GTP is converted into IMP with the enzyme GMP reductase. and this IMP converted into ATP.
from IMP it equally converted into GTP and ATP.
from Adenosine it converts into ATP but few molecules of Adenosine is converted back into IMP with AMP deaminase enzyme. and this IMP converted into GTP.
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