1. Using the Data given below answer the following questions la 1h. The V terms

Question

1. Using the Data given below answer the following questions la 1h. The V terms are all expressed in units of ?M/min and these experiments were carried out with 5 nM of the enzyme MMCmase The overall reaction catalyzed by MMCmase is: MM-CoA- C-CoA MM-CoA] (uM) | Velocity (AM/min) | Velocity (??/min) | Velocity (??/min) +5.5 mM EMC 0.75 mM CPC no additional molecules 0 45.5 105 216 576 691 0 12 72 144 300 0 17 40.5 91 356 505 650 0 70 158 384 1300 1990 2500 la. This enzyme is a mutase, what does that tell you about the substrate and product? (2 points) lb. This enzyme relies on the presence of adenosylcobalamin (a derivative of vitamin B12). What is type of molecule would you consider adenosylcobalamin to be for this reaction? NOTE: if you look this up please provide a reference along with your answer. (3 points)

Explanation / Answer

Ans. #1a. A mutase (intramolecular transferases) is the isomerases enzyme that transfers a group from one to another C-atom in the same molecule.

So, both the reactant and the product have the same chemical formula but different arrangement of atoms/groups. In other words, the reactant and product are constitutional isomer of each other.

#1b. Adenosylcobalamin acts as a coenzyme (organic cofactors) for this reaction. The small organic molecules, often derived from vitamins, that transiently associates with an apoenzyme to form catalytically active holoenzyme are called coenzymes. These organic cofactors mediate transfer of chemical groups, electrons or hydrogen during catalysis.

Ref: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3580769/

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