cysteine proteinase responsible for the digestion of seed storage proteins to gr
ID: 219122 • Letter: C
Question
cysteine proteinase responsible for the digestion of seed storage proteins to growth of young seedlings. In the present study, the gene encoding REP-1 wa designated as OsEP3.A. TheOsEP3 which encodes ?-glucuronidase (GUS), and the chimeric gene was introduced into the n of a Cysteine Proteinase Gene Expression in Rice. In many plants, cysteine proteinases play essential in a variety of developmental and physiological processes. In rice (Oryza sativa), REP-1 is a primary provide nutrients to support the isolated, characterized, and 3Apromoter was linked to the coding sequence of the reporter gene, gusA, rice genome. .What is the gene being expressed here? (4) What is the purpose of including rRNA in these lanes? What is your conclusion Lane 1 2 3 4 5 6 7 SEP3A RNA
Explanation / Answer
Since Gus gene is in the coding region, gus gene is being expressed here under the control of OsEP3A promoter.
rRNA is th internal control for loading here. this gene is expressed fairly equal by seedling and mature plant. so normalizing the band intensity of OsEP3A transcript with that of rRNA will help in the comparison of OsEP3A transcript expression difference between mature plant and seedling
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