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A genetic survey revealed that a population of spruce trees contained 2 alleles

ID: 218688 • Letter: A

Question

A genetic survey revealed that a population of spruce trees contained 2 alleles (B and b) for a gene affecting color of the needles. The survey of 10,000 individuals found the 3 possible genotypes in the following abundances:

(a) What are the frequencies of alleles B and b in this sample?

(b) How many of each genotype class would have been found if the population effectively

approximated the Hardy-Weinberg equilibrium?

(c) Estimate the value of the inbreeding coefficient (F) for this population given these data.

Genotype BB Bb bb Number found 4428 3604 1968

Explanation / Answer

a) Allele frequency estimation:

Genotype

Freequency

Allele B

Allele b

Total

BB

4428

8856

0

8856

Bb

3604

3604

3604

7208

bb

1968

0

3936

3936

Total

10000

12460

7540

20000

Allele frequency = Number of individual alleles/Total number of alleles…

Allele B

= 12460/20000

0.62

Allele b

= 7540/20000

0.38

b) Expected frequency estimation:

BB

=0.62*0.62 = 0.388

*10000= 3881

Bb

= 2 * 0.62 * 0.38 = 0.470

*10000= 4697

bb

= 0.38 *0.38 = 0.142

*10000= 1421

c) inbreeding coefficient = (# of Expected Heterozygotes - # of observed Heterozygotes) / (# of Expected Heterozygotes

= 4697-3604 / 4697 = 0.232

Genotype

Freequency

Allele B

Allele b

Total

BB

4428

8856

0

8856

Bb

3604

3604

3604

7208

bb

1968

0

3936

3936

Total

10000

12460

7540

20000

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