overlapping the argF promoter. Part of their evidence was a footprint on 11. A r
ID: 216984 • Letter: O
Question
overlapping the argF promoter. Part of their evidence was a footprint on 11. A regulatory protein binds a site the fa 29 base region, which extends from the middle of the -10 regi to somewhat upstream of the -35 region. Sketch this conclusion. Be sure to include a control (S) o ch the result of the footprint experiment that would lead to what is the region -10 to -35 called? What binds here? Will this protein be activator or repressor-? Explain your answer. Read about footprint experiment and explain how it is done .
Explanation / Answer
A promoter sequence of a bacterial DNA shows -10 and -35 regions. Promoter sequences could be seen on DNA. The regions -10 and -35 are consensus sequences in a promoter. These consensus sequences shows sequencely aligned frequent residues. -35 is TTGACA. -10 is TATAAT. Both -10 and -35 boxes could be seen at 10 bases and 35 bases upstream of transcription start site (+1). At these consensus sequences, the transcription factor binds to initiate mRNA transcription . Transcription factors are protein activators/repressors. The -10 and -35 concensus sequences can be identified only by RNA polymerase.
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