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During DNA replication in E. coli, the synthesis of each nascent strand occurs i

ID: 215826 • Letter: D

Question

During DNA replication in E. coli, the synthesis of each nascent strand occurs in a different way. Leading strand synthesis occurs continuously in the 5' to 3' direction. Lagging strand synthesis occurs discontinuously, seemingly in the 3' to 5' direction. In order to regulate the synthesis of both strands, some enzymes and molecules are specific to leading or lagging strand synthesis, whereas others are involved in both processes. Categorize each of the following enzymes or molecules based on whether it is required for leading strand synthesis, lagging strand synthesis, or both types of synthesis. Leading strand synthesis Lagging strand synthesis Both types of synthesis DNA gyrase DNA polymerase lII primase DNA polymerase I a single RNA primermultiple RNA primers ncorrect. Although other enzymes or molecules may be misplaced, you have specifically misplaced one or more of the factors that are required only for lagging strand synthesis. The lagging strand uses the formation of short Okazaki fragments that are later ligated together. Each Okazaki fragment requires a ribonucleotide primer

Explanation / Answer

This given answer is correct according to the process of replication in E. coli.

The enzymes/molecules involved are :

1.) Lagging strand synthesis- DNA Polymerase I, Primase, Multiple RNA Primers

2.) Leading strand synthesis- None

3.) Both Synthesis- DNA Gyrase, DNA Polymerase III, A Single RNA Primer

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