Final 2018 2 (20 pts) Below is a structural model of the ATP synthase also somet
ID: 214820 • Letter: F
Question
Final 2018 2 (20 pts) Below is a structural model of the ATP synthase also sometimes called the ATPase. A) (3 pts) From what you know about oxidative phosphorylation and how the ATP synthase works, where is the pH higher, in the space labeled 1 or the space labeled 2? B) (2 pts) The first researchers who studied this enzyme studied a soluble form of the enzyme that they prepared by cutting they- subunit at the membrane (dashed lines). This soluble form did not have the C-ring. Could this soluble form synthesize ATP? C) (12 pts) Use diagrams to explain HOW ATP is synthesized from ADP and Pi. Be sure to explain the role of the a, B. and y subunits including the aspartic acid in the C-ring and the role of the C-ring in the synthesis of ATP 2 D) (7 pts) Use similar diagrams to explain what happens in the ATPase reaction.Explanation / Answer
A. oxidative phosphorylation is a process of formation of ATP by the use of oxygen.
the electron transport system is the system for the transport of electrons as well as protons to produce energy in the form of ATP. The last electron acceptor is Oxygen and due to this acceptance of electron ATP is produced that is why it is called as oxidative phosphorylation.
ATP Synthase enzyme transfers phosphate group to adenosine diphosphate and forms ATP. ATP synthase is an membrane bound enzyme which have motor like shaft involved in rotation by Proton motive force. Due to the proton motive force a torque will generate and rotate the shaft of ATP synthase and produce ATP.
The mitochondrial Matrix have high number of protons due to this the pH in matrix is low.Space 2.
B.
No, C ring binds with H+ and rotates for the formation of ATP. So without seeing enzymes are not able to produce ATP.
C.
ATPases can harness the energy from a proton gradient, using the flux of ions across the membrane via the ATPase proton channel to drive the synthesis of ATP.
alpha and beta subunts are part of ATP synthase enzyme and involved in the rotation of ATP synthase partially by Proton motive force. C ring have aspartate residues which binds with protons and rotates the ring. Due to this rotation ADP binds with Pi and produce ATP.
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