1. How do you think the results would differ if you had used spinach leaves whic

Question

1. How do you think the results would differ if you had used spinach leaves which had been stored in a dark room for five days before the experiment? Explain your answer? (2 points)

2. The spinach leaf looks green, but your chromatogram demonstrated that other pigments are in the leaf as well. Why can you not readily see the other pigments in the leaf? (2 points)

3. Why is wavelength information of value in studying plants? (2 points)

4. In which colors of light would you expect a plant to obtain maximum photosynthetic activity? (2 points)

5. What does the absorption spectrum convey about each pigment? Be specific with details. (5 points)

Explanation / Answer

1) When plants are deprived of light they, chlorophyll gradually breaks down and can not be replenished. If the spinach leaves used were stored in darkroom for 5 days, then they will have less chlorophyll content present in the extract and much lower peaks in absorption specturm could be expected.

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