Figure 7.12 shows the results of two restriction digests and subsequent electrop

Question

Figure 7.12 shows the results of two restriction digests and subsequent electropho- resis, each one analyzing two DNA samples. In Analysis 1, the profiles match. In Analysis 2, the profiles do not match. The DNA fragments have been numbered for reference for the questions that follow 150 5. Cathode (black) 10 10 12 13 13 Sample Sample Sample Sample 8 A. Analysis 2 Anode (red) Analysis 1 Anode (red) Figure 7.12. Two RFLP analyses. List the fragments (by number) in Analysis 2 that do not match between Sample A and Sample B. Which one is the lon- Which fragment in Analysis 2 is the shortest fragment? gest? Which fragment in Analysis 1 has the most repeating sequences in the introns? 16. There are two types of karyokinesis: mitosis and meiosis. When a nucleus divides by mitosis, how much of the information from the nuclear chromosomes is passed on to the daughter cells?

Explanation / Answer

Question: List the fragment in analysis 2 that do not match between sample A and sample B

Answer: fragment number 2,3,7,8,9,10,12 and 13 .

Quesrion :which fragment in analysis 2 has the shortest fragment and which one is the longest

Answer: shortest fragment is 13 and longest fragment is 1.

Question : 6

Answer: During mitosis there is complete transfer of genetic information in the doughter cells .

If a parent has 2n chromosome number then doghter cells will also have 2n chromosome number .

There is no reductional division so that a complete transfer of genetic material takes place in doughter cells .

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