Figure 7.12 shows the results of two restriction digests and subsequent electrop

Question

Figure 7.12 shows the results of two restriction digests and subsequent electropho- resis, each one analyzing two DNA samples. In Analysis 1, the profiles match. In Analysis 2, the profiles do not match. The DNA fragments have been numbered for reference for the questions that follow. 5. Cathode (black) Cathode (black) 10 10 12 - 12 13 Sample Sample Sample Sample Analysis 2 +Analysis 1 (red) (red) Figure 7.12. Two RFLP analyses. List the fragments (by number) in Analysis 2 that do not match between Sample A and Sample E. which fragment in Analysis 2 is the shortest fragment?--which one is the lon- gest? Which fragment in Analysis 1 has the most repeating sequences in the introns?

Explanation / Answer

1) The fragments that do not match between sample A and sample B in analysis 2:

Fragment number: 2, 3, 7, 8, 9, 10, 12, 13

2) In analysis 2, shortest fragment is fragment 13, the longest fragment is fragment 1.

3) Fragment 5, 6 have the most repeating sequences in the introns.

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