4a. Fill in the table below with the missing information about the protein Czr2p

Question

4a. Fill in the table below with the missing information about the protein Czr2p, include proper units if needed Ligand AMP CMP GMP UMP ki (M sec 30 0.01 k.i (sec) 1.5 X 10 Kd 0.9 mM 85 LLM 5.1 X 10 2 X 10 0.5 4ai. Circle the ligand that Czr2p has the lowest affinity for 4b. The Czr2p protein is a trimer (made up of three subunits) with all subunits being the same If you determined that the protein-ligand binding data for Czr2p with GMP the Hill coefficient (or n) is 3, what does this indicate about this protein's ability to bind the GMP ligand, specifically 4bi. Do you think Czr2p binds to more than one molecule of GMP? 4bii. Does Czr2p with the GMP ligand act in a positively cooperative manner, a non-cooperative manner or in a negatively cooperative manner? Explain what your choice here means for the Czr2p protein and the GMP ligand.

Explanation / Answer

Kd= k_1/k1

4a.

1. Kd = 5× 10^-6

2.k_1=9×10^-3

3.k1= 6

4. Kd= 4 × 10^-4

4a1. CMP has lowest affinity

4b. Hill cofficient measure of cooperativity in a binding process. A Hill coefficient of 1 indicates independent binding, a value of greater than 1 shows positive cooperativity binding of one ligand facilitates binding of subsequent ligands at other sites on the multimeric receptor complex.

Since protein czr2p has hill coefficient 3 mean it can bind more than 1 ligand with positive cooperation.

4b 1. Yes it will bind more than one molecule og GMP.

4b2. GMP will act in Positive cooperative manner

Related Questions

Navigate

• Browse (All)
• Browse 4
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.