Find the primer binding sites and then calculate the size of the PCR product Pri

Question

Find the primer binding sites and then calculate the size of the PCR product Primer 1 5'- ctccaggagtcg -3' Primer2 5'- tgcacggcagatac -3' 1 ttatttttga caccagacca actggtaatg gtagcgaccg gegctcagct ggaattccgc 61 cgatactgac gggctccagg agtcgtcgcc accaatcccc atatggaaac cgtcgatatt 121 cagccatgtg ccttcttccg cgtgcagcag atggcgatgg ctggtttcca tcagttgctg 181 ttgactgtag cggctgatgt tgaactggaa gtcgccgcgc cactggtgtg ggccataatt 241 caattcgcgc gtcccgcagc gcagaccgtt ttcgctcggg aagacgtacg gggtatacat 301 gtctgacaat ggcagatccc agcggtcaaa acaggcggca gtaaggcggt cgggatagtt 361 ttcttgcggc cctaatccga gccagtttac ccgctctgct acctgcgcca gctggcagtt 421 caggccaatc cgcgccggat gcggtgtatc gctcgccact tcaacatcaa cggtaatcgc 481 catttgacca ctaccatcaa tccggtaggt tttccggctg ataaataagg ttttcccctg 541 atgctgccac gegtgagcgg tcgtaatcag caccgcatca gcaagtgtat ctgccgtgca

Explanation / Answer

Primers will bind at the Bolded Portion

ttatttttga caccagacca actggtaatg gtagcgaccg gcgctcagct ggaattccgc

cgatactgac gggctccagg agtcgtcgcc accaatcccc atatggaaac cgtcgatatt

cagccatgtg ccttcttccg cgtgcagcag atggcgatgg ctggtttcca tcagttgctg

ttgactgtag cggctgatgt tgaactggaa gtcgccgcgc cactggtgtg ggccataatt

caattcgcgcgtcccgcagc gcagaccgtt ttcgctcggg aagacgtacg gggtatacat

gtctgacaat ggcagatccc agcggtcaaa acaggcggca gtaaggcggt cgggatagtt

ttcttgcggc cctaatccga gccagtttacccgctctgct acctgcgcca gctggcagtt

caggccaatc cgcgccggat gcggtgtatc gctcgccact tcaacatcaa cggtaatcgc

catttgacca ctaccatcaa tccggtaggt tttccggctg ataaataagg ttttcccctg

atgctgccac gcgtgagcgg tcgtaatcag caccgcatca gcaagtgtat ctgccgtgca

The PCR Product size will be of 603 bp

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