Lab: DNA GeLElectronhoresis(Part 4 A B CDEF 900bp 800bp G H I J K L 450bp 50b 10
ID: 210015 • Letter: L
Question
Lab: DNA GeLElectronhoresis(Part 4 A B CDEF 900bp 800bp G H I J K L 450bp 50b 100b Instructions: The above image is of a completed DNA gel containing the fragmented DNA left at the scene of a brutal double homicide. Four suspects were eventually apprehended and their respective DNA samples occupy lanes/wells 1-4 of the gel. Please answer the following questions completely and to the best of your ability and attach with your respective lab report. 1. Identify the specific lane/well of the gel loaded with the DNA standard/ladder. 2. Lane 5 holds the DNA left at the scene of the crime. Lanes 1-4 are the DNA samples of the suspects. Identify the suspect or suspects that was/were present at the scene of the crime. Does this mean that the identified suspect(s) is guilty of said crime? Explain your rationale. 3. Approximate the size of each fragment of DNA within the positively identified suspect's sample ONLYExplanation / Answer
1. A and L. They are running two different gel electrophoresis so they used two ladders.
2. Lane 2 is most similar band with lane 5. So he might be suspect in this crime scene.
3. 900, 850, 800, 300, 295, 275, 270, 175, 100, 95 kb.
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