Lab worksheet 4 Module C Due in class week of Sep 26. TA/Section: Now that you k
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Question
Lab worksheet 4 Module C Due in class week of Sep 26. TA/Section: Now that you know how to use each of the tools above, answer each question to the best ofyour ability. Show ALL calculations and use complete sentences. One-word answers will never be given credit. Use the UCSC Genome Browser to bring up the TAS2R38 gene in the browser and answer each of the following: 1. What chromosome is the gene on? (0.25 points) What are the start and stop sites for transcription? (0.25 points) What is the size of the coding region? (0.5 points) a. b. c. Click on the blue line that represents the gene to find each of the following pieces of information: 2. What is the subcellular location of TAS2R38 protein (look under Comments and Description)? (0.25 points) a. b. What tissues show TAS2R38 expression? (0.25 points) c. What other chemicals interact with this gene, other than PTC (look under Comparative Toxological Database)? (0.5 points) 3. Using the in silico PCR result a. What chromosome is the PCR product on? (0.25 points) b. What is the genome position of the PCR product? (0.25 points) c. What is the size of the PCR product? (0.25 points) d. Is the PCR product closer to the beginning or end of the TASZR38 gene? (Remember that the gene runs right to left as drawn). (0.25 points)Explanation / Answer
1)
a) The gene is located on chromosome 7. The cytogenetic location is 7q34, which is the long (q) arm of chromosome 7 at position 34.
b) It spans from base pairs 141,972,631 to 141,973,773 on chromosome 7 (Homo sapiens Annotation Release 109, GRCh38.p12)
c) The coding region is 1001 nucleotides long and codes for a protein which has approximately 333 amino acids.
2)
a)The gene TAS2R38 encodes a seven-transmembrane G protein-coupled receptor. It is a transmembrane protein, located/spans the membrane. that controls the ability to taste glucosinolates.
b) It is present in human gustatory tissues/ human taste cells, whole blood, spleen , small intestine.
3) No forward and reverse primer sequence provided for answering in silico PCR question.
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