Lab titled Creatinine Determination: Picric acid Reaction with Creatinine In thi
ID: 589210 • Letter: L
Question
Lab titled Creatinine Determination: Picric acid Reaction with Creatinine
In this assay you will determine the concentration of creatine in an unknown sample, using a colorimetric assay. The reaction performed in this lab experiment is known as Jaffe Reaction c and it is the most popular method for creatine determination.
What is the function of picric acid in creatinine determination? How is excess creatinine excreted from the body? Total urinary creatinine is calculated by multiplying the creatinine concentration by the total volume of urine over 24 hours. What is the range of the value in normal adults (Male and Female)?Explanation / Answer
1. Picric acid reacts with the creatinine in alkaline medium and forms a red coloured complex, and its absorbance is proporional to the creatinine concentraion. for this purpose it is used.
2. usually kidneys filter creatinine from blood, and then passed out of body through urine.
3. normal urinary creatinine value ranges from 955-2936 mg for 24 hrs in male and 601-1689 mg in female for 24 hrs
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