Lactobacillus acidophilus is a gram-positive bacterium commonly used in yogurt p

Question

Lactobacillus acidophilus is a gram-positive bacterium commonly used in yogurt production. It is an obligate homofermentative bacterium, which means that it is only capable of carrying out fermentation metabolism to produce lactic acid. It is also an aerotolerant anaerobe, which means it is able to tolerate atmospheric oxygen concentrations but does not use oxygen in its metabolic processes. Describe the results you would expect to obtain if you tested this bacterium using the oxidase test, nitrate reduction test, EMB agar test, and blood agar test. For each, explain the reason you would expect to observe that result.

Explanation / Answer

Lactobacillus acidophilus will not produce cytochrome C oxidase so it will be oxidase negative. It will not reduce the nitrogen so it is also negative on nitrate reduction test. As it farments and produce acid so it produce a metalic green sheen colonies on EMB agar. As it does not causes any hemolysis so it will not show any hemolysis in blood agar

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