PART 4 Genomics of Spore Color in Sordaria macrospora Access to the Ensemble fun

Question

PART 4 Genomics of Spore Color in Sordaria macrospora Access to the Ensemble fungi database to identify three genes that are involved in the synthesis of melanin in S. macrospora and therefore most likely associated with its ascospore color. For each of the three identified genes, briefly describe its transcript (total length, presence of introns and exons), its protein (number of amino acids), its biological function(s) and its locus. Gene TranscriptProtein Biological function(s)Gene locus (location) 2.18:297386-298127 SMAC_02101 CCC11445 Scytalone SMAC_ 03698CCC09665 Tyrosine SMAC-007 12 | CCC06686 | Homogentisate (174aa) SuperContig 2.9:927,562-929,976 forward strand (582aa) (470aa) SuperContig 2.2:304,669-306,406 forward strand Briefly explain how the gene location is defined in the genomics database of S. macrospora. How many possible spore color mutants might be characterized in S. macrospora?

Explanation / Answer

1) Protein Scytalone 174aa : Biological function: Melanin biosenthetic process .Exons: 3, Coding exons: 3, Transcript length: 619 bps, Translation length: 174 residues

(link provided):http://www.uniprot.org/uniprot/F7W0Z3

2) Protien tyrosine 582aa: Biological function: Catalytic activity :Catalysis of the reaction: protein tyrosine phosphate + H2O = protein tyrosine + phosphate. Exons: 2, Coding exons: 2, Transcript length: 2,334 bps, Translation length: 582 residues

(link provide) https://www.ebi.ac.uk/QuickGO/term/GO:0004725

3) Protein Homogentisate 470aa : Biological function: involves in phenylalanine catabolic process and tyrosine metabolic process. Exons: 2, Coding exons: 2, Transcript length: 1,679 bps, Translation length: 470 residues

(link provide) http://www.uniprot.org/uniprot/F7VMV7

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