23. The limit of resolution of a light microscope is a function of A. the magnif

Question

23. The limit of resolution of a light microscope is a function of A. the magnifying power of the lenses B. the numerical aperture of the lens C. the wavelength of light used D. Both A and B E. Both B and C 24. The optimal temperature for most human pathogens might be expected to range from A. 35-40°C B. 20-45°C. C. 15-25°C. D. 93-98.6°C True or False? Switching from high dry objective to oil immersion it is advisable to place a drop of oil on the slide and reduce the light by closing down the diaphragm. 2 26. True or false? The coarse adjustment knob can be used to adjust the focus when using the oil immersion lens 27. True or False? Once focus is achieved at high dry magnification it is advisable to lower the stage when rotating to oil immersion lens to avoid striking the slide. 28. Staining is necessary to visualize cells using the light microscope. Which of the following use of the light microscope can be done without cell staining? A. Phase contrast. B. Dark field C. Bright field. D. Fluorescent. E. Both A and B

Explanation / Answer

23. E, Most important factor in resolving the image is numerical aperture of the microscope. The shorter wavelengths generally produce higher resolving power.

24. A, the human body temperature is 37 degree Celsius hence the pathogens inhabitating have range 35 to 40 degree Celsius.

25. True, Adjust the light using diaphragm.

26. False, coarse knob should not be used in focusing the slide, instead fine adjustment knob should be used when dealing with higher magnifications.

27. False, it will change the focus of the slide and you have to re adjust it. But be careful sometimes silde comes very close to lens.

28. Both A and B, phase contrast and dark field microscopy can be used.

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