1. put the steps for constructing a genomic library in the correct order step1 s

Question

1. put the steps for constructing a genomic library in the correct order

step1

step 2

step 3

step 4

step 5

a. use a ligase on the DNA and the vector

c. Transform host bacteria

d. use a restriction on the DNA and the vector

e. isolate mRNA from the organism

f. culture bacteria on a plate containing antibiotics

g. use reverse transcriptase on the DNA and the vector

2. In term of size, a genomic library is...................... a. larger b. smaller Than a cDNA library. A................................. a. genomic b. cDNA the library cannot be used to express proteins in a bacterial host because it contains introns. cDNA libraries constructed from different tissues are expected to contain.................. a. different b. identical 5. In a DNA library, each plasmid can contain more than one gene. This is not true for a cDNA library because a. one molecule of cDNA only contains one gene b. Different restriction enzymes are used c. Host cells cannot be transformed when there is more than one gene in the plasmid d. the vector used in cDNA libraries are different.

4. which technique allows you to identify a specific DNA or RNA sequence of interest?

a. complementary probe

b. Gel electrophoresis

c. Western blot d. restriction digestion

3. which of the following statements is (are) true about restriction enzymes?

a.discovered in bacteria

b. Cleave the phosphodiester in the backbone

c. Cleave the peptide bond in the backbone

d. produces random fragments

e. recognizes specific segments

11. you wish to create a transgenic mouse that is completely hairless. You introduce the appropriate mutation into the embryonic stem cells and implant those cells into early embryos. Those embryos are placed in a surrogate mouse who then gives birth to the first generation of mice. These mice are further bred. Which of the following is (are) true?

a. first generation is completely hairless

b. The second generation is completely hairless

c. The third generation is completely hairless

d. the second generation is heterozygous

e. the second generation are crossed with each other

f. the first generation are crossed with each other

Explanation / Answer

1.

Step 1: Isolate DNA from the organism

Step 2: Use a restriction on the DNA and the vector

Step 3: Use a ligase on the DNA and the vector

Step 4: Transform host bacteria

Step 5: Culture bacteria on a plate containing antibiotics

2. In term of size, a genomic library is larger than a cDNA library. This is because the entire genome does not code for RNA.

3. A genomic library cannot be used to express proteins in a bacterial host because it contains introns. cDNA libraries constructed from different tissues are expected to contain different cDNAs.

4. The complementary probe allows you to identify a specific DNA or RNA sequence of interest?

5. In a DNA library, each plasmid can contain more than one gene. This is not true for a cDNA library because one molecule of cDNA only contains one gene

Related Questions

Navigate

• Browse (All)
• Browse 1
• Subjects

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.