1. put the steps for constructing a genomic library in the correct order step1 s
ID: 205327 • Letter: 1
Question
1. put the steps for constructing a genomic library in the correct order
step1
step 2
step 3
step 4
step 5
a. use a ligase on the DNA and the vector
b. Isolate DNA from the organism
c. Transform host bacteria
d. use a restriction on the DNA and the vector
e. isolate mRNA from the organism
f. culture bacteria on a plate containing antibiotics
e. use reverse transcriptase on the DNA and the vector
2. In term of size, a genomic library is......................
a. larger
b. smaller
Than a cDNA library. A.................................
a. genomic
b. cDNA
the library cannot be used to express proteins in a bacterial host because it contains introns. cDNA libraries constructed from different tissues are expected to contain..................
a. different
b. identical
5. In a DNA library, each plasmid can contain more than one gene. This is not true for a cDNA library because
a. one molecule of cDNA only contains one gene
b. Different restriction enzymes are used
c. Host cells cannot be transformed when there is more than one gene in the plasmid
d. the vector used in cDNA libraries are different
4. which technique allows you to identify a specific DNA or RNA sequence of interest?
a. complementary probe
b. Gel electrophoresis
c. Western blot
d. restriction digestion
2. which of the following statements is (are) true about restriction enzymes?
a.discovered in bacteria
b. Cleave the phosphodiester in the backbone
c. Cleave the peptide bond in the backbone
d. produces random fragments
e. recognizes specific segments
11. you wish to create a transgenic mouse that is completely hairless. You introduce the appropriate mutation into the embryonic stem cells and implant those cells into early embryos. Those embryos are placed in a surrogate mouse who then gives birth to the first generation of mice. These mice are further bred.
Which of the following is (are) true?
a. first generation is completely hairless
b. The second generation is completely hairless
c. The third generation is completely hairless
d. the second generation is heterozygous
e. the second generation are crossed with each other
f. the first generation are crossed with each other
Explanation / Answer
as per chegg guidelines if you post more than 1 ques i have to answer only one question
ques4-- COMPLEMENTARY PROBE helps to identify a specific RNA or DNA sequence of interest .
it is basically a technique in which a complementary sequence of DNA can be identified, from a DNA strand using a known sequence of nucleotide bases by means of base pairing.
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