Figure 2 below is a drawing that illustrates part of the experiment done above.

ID: 203281 • Letter: F

Question

Figure 2 below is a drawing that illustrates part of the experiment done above. The test tube on the left contains enzyme but no substrate at pH 8 (control). The next tube contains enzyme with the highest concentration of substrate shown in the graph in Figure 1, also at pH 8.

I. CONTROL II. pH 8 III. TUBE A IV. TUBE B

a) Which point on the results graph above (Figure 1) corresponds to test tube II at pH 8? (you can describe which point by its placement on the X and Y axis, and which curve it is found on; or just upload a picture with it circled).

b) For the two remaining test tubes on the right (III. Tube A and IV. Tube B), draw in the experimental conditions that correspond to points A and B on the graph in Figure 1. Be sure to draw the enzyme and substrate bound or unbound at the appropriate concentrations as in the control and pH 8 tubes (I and II).

c) Which of the pH values tested in this experiment is most ideal for the action of the enzyme? How can you tell?

pH 5 4 pH 6 pH 7 pH 8 Substrate concentration

Explanation / Answer

a] first point on the curve for ph 8

b] The amount of bound product in tube two is more than tube 1 since tube

ph7 is most ideal for the enzyme

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Figure 2 below is a drawing that illustrates part of the experiment done above.

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