Why is important to have replicates in a scientific study? .\'ll AT&T; 11:17 PM

Question

Why is important to have replicates in a scientific study? .'ll AT&T; 11:17 PM clayton.view.usg.edu is a yeast species that has catalase enzymes to protect it from toxic byproducts of acrobic metabolism. Scientists know that the pH of the environment can affect an enzyme's activity. Because of this, a group of students has designed an experiment to test the effect of pH on catalase activity. Using the same methods we devised in class (using the time it takes for a disc to float as an indicator of catalase activity the longer it takes to float, the less active catalase is), the students set up an experiment where they test catalase activity at a neutral pH (pH = 7) and at an acidic pH (pH = 4). The compared the reaction rates from each group to see if there was an effect. . What is the independent variable in this study? Is this variable quantitative or categorical? What is the dependent variable in this study? Is this variable quantitative or categorical? What is an appropriate hypothesis for this experiment? What is an appropriate prediction for this experiment? s. Identify the different treatment groups. If there is a control treatment group, identify it. 6. List two potentially confounding variables that the student would need to control. 7. Why it is important to control potentially confounding

Explanation / Answer

Let us understand the experiment first,

1. Cut the paper into small discs, dip it in a yeast solution then leave the disc in a tissue towel for 1 min to drain/dry excess of yeast( source of catalase enzyme).

2. Take H202 substrate solution in a cup, put the yeast dipped discs to the bottom of the H202 substrate solution and start the stopwatch.

3. Record the timings when discs starts to rise from the bottom of the cup.

Qsn1: Independent variable could be the size of filter disc

Qsn2: temparature, pH, concentration of H202 substrates, yeast solution ( source of catalse enzyme) all are dependent variable and can be quantitative.

Qsn3 & 4 : Amount of time taken indicates the efficiency(faster) the enzyme activity is. Your catalase enzyme will convert 2H202 -->2 H20+o2. Catalase enzyme is essential in removing of toxic bi-products like (H202) from our body and though by prevents tissue damage during stress condition.

Qsn5: treatment groups: concentration of H202, percentage of yeast solution.

Qsn6: Your H202 solution and yeast solution should be freshly prepared and the amount of time taken to perform this experiment should be kept in mind has variation in any of these things can leads to variations in your experimental results.

Qsn7: H202 when exposed to light can undergo catalytic reaction to convert intoH20 & O2 which may give wrong interpretation of your results. Longer the duration is your catalase enzyme will start loosing its enzyme activity.

Qsn: Average data of any experiment repeated together will minimize the variability in any experiment.

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