Website link: http://www.addgene.org/browse/sequence_vdb/1979/ Map and Feautures

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Website link: http://www.addgene.org/browse/sequence_vdb/1979/

Map and Feautures

Questions:

1)If we used restriction enzymes to remove just the Ampicillin resistance gene, how large of a fragment would we see? (Ampicillin gene labeled)

2) If we used a restriction enzyme that cut the plasmid one time, how large of a fragment would we see?

I pasted the link I think I have an idea, but this is graded so I need help!

Addgene - Anelyze Siqua agtggtcctg gaagctaga ccattgctgc ttcagctccg tgcaaaaaa cgcctccatc gccagttaa gtgtcacgct atcaaggcga ccttcqgtcc cagtctatta tagtttgcgc cgtcgtttg caactttatC taagtagtt 3551 3601 3651 3701 aggcatcgt ttcccaac cagttagct ttacatgat tccgatcatt Map and Features Sequence Blast Align Digest Translate Circular Map | Linear Map pBR322: 4361 bp Clal (24) Hindill (29) EcoRV (187) Nhel (229) BamHl (375) ORF frame Sall (651) ROP Asel (3538) l (3611) Ndel (22a6) 4000 Macl (1 Aatil (4288) Feature Options Restriction Enzymes Unique 2-cutters hide all Show Label Feature Type Generic features Genes

Explanation / Answer

A- Upon digestion of ampicillin resistance gene with the restriction enzyme, we will get the fragment of 860 bp.

B- given that the total length of the vector pBR322 is 4361 bp. So upon digestion of circular vector with the restriction enzyme which has only one site in the vector, we will get a fragment of 4361 bp on the gel.

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