Figure 1: Figure 2: In the figure 2 , there is a band at 12,275-bp for the BamHI

ID: 197972 • Letter: F

Question

Figure 1:

Figure 2:

In the figure 2 , there is a band at 12,275-bp for the BamHI cleaved lambda DNA and a 24,756-bp band for the EcoRI cleaved lambda DNA even though those fragments do not appear on the lambda DNA restriction map in Figure 1. Explain why this is the case.

DNA 0 10,000 20,000 30,000 40,000 (bp) 48,502 5,505 16,841 6,527 7,233 5,626 6,770 BamHl 5,505 22,346 27,972 34,499 41,732 21,226 4,878 5,643 21,226 26,104 31,747 |7,421 5,804 |3,530 EcoRI 39,1168 44,972 2,027 564 2,322 125 23,130 9,416 6,557 4,361 Hindlll 23,130 27,479 25,157 36,895 37,584 44,141 37,459 Figure 1

Explanation / Answer

This is because the lambda DNA is circular. And the restriction map is showing circular DNA.

Note the two extremes of BamH1 DNA have the cuts: (5505+6770)= 12275bp

Similarly the two extreme ends of EcoR1 linear DNA have the bands: (21226+3530)= 24756bp

Bands of cut circular DNA have been shown in figure 2.

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Figure 1: Diagram showing the physical situation of a line of charge with a unif

Figure 1: Figure 2: Relevant Information concerning GW: Answers for Questions 2-

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Figure 1: Figure 2: In the figure 2 , there is a band at 12,275-bp for the BamHI

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