You discover a new methanogenic (methane-producing) bacterial species and want t

Question

You discover a new methanogenic (methane-producing) bacterial species and want to determine if it produces its ATP by substrate level phosphorylation or by electron transport/chemiosmosis. After determining the amount of ATP made under normal conditions, you treat cells with tetrachlorosalicylanilide (TCS). TCS is an uncoupler that shuttles protons across membranes in both directions. Treatment with TCS leads to a dramatic reduction in ATP synthesis. Based on the results of this experiment, what can you conclude about how your newly discovered methanogen produces ATP under normal conditions? produces most of its ATP by fermentation produces most of its ATP by substrate-level phosphorylation produces most of its ATP by electron transport/chemiosmosis generates a proton gradient that is coupled to ATP synthesis requires oxygen to produce ATP

Explanation / Answer

Production of ATP occurs when the proton or electrochemical gradient generated is coupled to phosphorylation of ADP to synthesize ATP. This process seen in both Substrate level phosphorylation and ETC also which is inhibhited by UNCOUPLER, they inhibhit this coupling and prevent ATP generation

Further methanogenic bacteria derives it energy in anoxic condition , So the most apt answer here is :

generates a protein gradient that is coupled to ATP synthesis

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