You discover a new methanogenic (methane-producing) bacterial species and want t

Question

You discover a new methanogenic (methane-producing) bacterial species and want to determine if it produces its ATP by substrate level phosphorylation or by electron transport/chemiosmosis. After determining the amount of ATP made under normal conditions, you treat cells with tetrachlorosalicylanilide (TCS). TCS is an uncoupler that shuttles protons across membranes in both directions. Treatment with TOE leads to a dramatic reduction in ATP synthesis. Based on the results of this experiment, what can you conclude about how your newly discovered methanogen produces ATP under normal conditions? produces most of its ATP by fermentation produces most of its ATP by substrate-level phosphorylation produces most of its ATP by electron transport/chemiosmosis generates a proton gradient that is coupled to ATP synthesis requires oxygen to produce ATP

Explanation / Answer

Answer:

Addition of phosphate group to any one of the substrate is called phosphorylation. Substrate level phosphorylation involves formation of ATP by physical addition of phosphate group to adenosine diphosphate (ADP) or guanosine diphosphate (GDP). Substrate level phosphorylation does not involve the electron transport chain and the resultant proton motive force to generate ATP. Hence, when uncouplers like TCS (tetracholorosalicylanilide) are used to produce ATP by substrate level phosphorylation.

Hence, correct option is produces most of its ATP by substrate-level phosphorylation.

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