46 DNA Replication Exercise B: DNA Replication (con\'t) SSBP tO- Cytosine td- Ad

Question

46 DNA Replication Exercise B: DNA Replication (con't) SSBP tO- Cytosine td- Adenine t - Helicase GuanineDNA Polymerase I . Thymine @-DNA Polymerase ll , Uracil . . DNA Polymerase III . Deoxyribose . Primase . Ribose A-Ligase -Phosphate 4h. Telomerase ( In Fig. 7, label the leading and lagging strands in the boxes. Fig. 7. Lagging and leading strands during the elongation phase of DNA replication. Elongation stage of replication: leading strand vs. lagging strand (con't) On the opposite strand a problem arises due to the antiparallel nature of DNA and the fact that DNA Polymerase Ill can only add base pairs in the 3' direction.SSBPs must be added to prevent the parental DNA strands from reattaching, primase has to attach another RNA primer and DNA Polymerase I" has to add D-nucleotides in the reverse direction as the helicase is traveling. This disrupts the addition of D-nucleotides by DNA polymerase III, causing the initiation and elongation steps of DNA replication to repeat multiple times. This discontinuously generated new DNA strand is the logging strand (Fig. 7) Once primase matches up the R-nucleotides that make up the primer, it is automatically removed U A UA C9 0- 5" 2 Draw the new DNA nucleotides. Draw the new DNA nucleotides. when DNA Polymerase III comes in contact with the Fe- 8. Elongation phase of replication. SSBPs, it removes them

Explanation / Answer

a. as indicated in figure the upper strand which is going toward replication for, is a leading strand, while the lower one which is going away from replication fork is lagging strand

in the figure two the complementory nucleotides for upper box which hase nucleotide C, T, T, C, T, A is

                                                                                                                          G, A, A,G, A,T

and in the lower box A, T, G, C,C, G is

                              T, A, C, G,G, C

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