DESIGN PCR PRIMER FOR THE FOLLOWING SEQUENCE 50% G/C, AT LEAST 20 nt in leght Ca

Question

DESIGN PCR PRIMER FOR THE FOLLOWING SEQUENCE 50% G/C, AT LEAST 20 nt in leght

Calculate each primer Tm .

1 ATACACTTAAACTTCTTTGCAAAACTTTCATCAAAATATTATATTTAGTATGAATTCCGC

                                                                                                                  Start codon

61 TCGGTTCTACGCTCAATACAGCATGTCCCGTCTCGTAAATAGTGTGACTACTTTTAAGAG

121 TAGTGGCGAATCTTGCAAGTTGCATCGAATTTACAAAATTAGTTTTCCTTCGGGTCAAAA

                                                                 

181 GTATATTGGACAAACCGTGAAAATGCCCAATGAACGCCTCAAGGAACACCAACGAGAATC

                                                                 

241 ATCCAAGTGTCATAGGCTAAAACACGCTCTCCGAACAAATCCTGATTTCAAATTAGACAC

301 GGTCGCGATTGTAGGACCTCACCAGGTTCATGCTGTAGAAAAAATTGCCATTGCTCTCGA

361 AGATTCAACAGGTCCAAACGGCCTTAACATAACCATCGGGGGTGCTGGAGTGAAGAAGCC

                                                                 

421 AGATGAAAGATACGAAACTTACAAGAAGCACGTTAGTGATATATATCATCGTATGAAGAG

                                                                 

481 AGGATCTTTCGTATCATACGACGTTTTGTTCATGAAAAATGATACTATGCTATCCGAAGA

                                                                 

541 AGAATTTCAAGCCATCAGACGGCTTTTGTAAAATCACCGGGATTCTAATAATTTCACACG

  

601 TTCTTCTAGAGTTTCAAGAGA

Explanation / Answer

Primer pair 1

Primer pair 2

Primer pair 3

Primer pair 4

Primer pair 5

Primer pair 6

Primer pair 7

Primer pair 8

Primer pair 9

Primer pair 10

Sequence (5'->3') Template strand Length Start Stop Tm GC% Self complementarity Self 3' complementarity Forward primer ATACAGCATGTCCCGTCTCG Plus 20 84 103 59.61 55.00 4.00 2.00 Reverse primer TTGGTGTTCCTTGAGGCGTT Minus 20 240 221 60.11 50.00 4.00 0.00 Product length 157

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