an ecori fragment of mouse DNA carries the gene M an ecori fragment of mouse DNA
ID: 189516 • Letter: A
Question
an ecori fragment of mouse DNA carries the gene M an ecori fragment of mouse DNA carries the gene M restriction enrymes EeoRL, Pa and Bamll, perfonning agarose gel electrophoresis and staining the DNA for visualization? A Lane D B. Lane C Lane B Lane A Standard D. Figure 3 S2W12. An EcoRI fragment of mouse DNA carries the gene M. This DNA fragment, which is 8 kb long, is inserted into the bacterial plasmid pBR 322 at the EcoRl site. A Southern blot is prepared from a gel in which the recombinant plasmid was cut with Baml and EcoRI. In the Southern blot, which of the following fragment sizes will hybridize to a radioactivePa probe of pBR 322 plasmid DNA? Sspt a Pvl 37 exl 712 A 2.5 kb fragment B. 1.0 kb fragment C. 0.4 kb fragment o311 3433 11058 230 pBR322 4361 bp 3.0 kb fragment E 4.0 kb fragment a12001 13 co1308 13 Call 2884 .epu o.sao 080 Kpnzi 1804 Psct 242 Pull 2064 2117 Espa 2 72 t1 1071 2244Explanation / Answer
the distance between ecor1 and bamh1 site is approximately 370bp in plasmid which is removed as a different band when double digestion is performed. the gene M is removed as it is as it has ecor1 site on both of its ends and again show itself as seperate band.
the remaining band of around 4kb that is majourly plasmid remains and can be used as a target for probe against pbr322 and hence answer is D) 4kb
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