The figure to the right is a diagram of the \"fingerprint obtained by subjecting

Question

The figure to the right is a diagram of the "fingerprint obtained by subjecting a mixture of 18 of the 20 common amino acids (all except Asn and Gln) to electrophoresis in one dimension and chromatography in the other (butanol:acetic acid:pyridine). The original spot containing all 18 amino acids was at the origin. During electrophoresis, similar sized molecules move primarily due to differences in their net electrical charge, with anions moving toward the anode (positive terminal) and cations moving toward the cathode (negative terminal). During chromatography in this system, molecules move primarily due to differences in polarity, with more polar molecules moving less and less polar molecules moving more. Identify the numbered spots. They correspond to the amino acids Arg Asp, Cys, Glu, His, Ile, Leu, Lys, and Pro. (Hint: There are 9 amino acids, but only 8 spots!) (0.5 pt each) Brown Yellow Origin Spot number 1: Chromatography Spot number 2: Spot number 3: Spot number 4: Spot number 5: Spot number 6: Spot number 7: Spot number 8:

Explanation / Answer

the amino acids can be the identifided by chromatography metod by the Rf of the amino acid , to find the Rf of amino acid use the formula

Rf= distance traveled by aminoacid/ the distance traveled by the solvent front

As per the marking of spots along the chromatography paper comparing the therotical Rf value i have answerd

Spot number 1)- Lys -0.14 Rf

Spot number2) -Cys-0.4Rf

Spot number3)-Pro-0.43Rf

Spot number4)-Glu-0.13Rf

Spot number5)- Arg-0.20Rf

Spot number6)-His--0.11Rf

Spot number7)-Asp-0.5Rf

Spot number8)it may be either Ile/Leu -0.72/0.73Rf

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