37. The pK1, pK2, and pKR of the amino acid histdine are 1.8, 9.3, and 6.0, resp

Q: 37. The pK1, pK2, and pKR of the amino acid histdine are 1.8, 9.3, and 6.0, resp

The correct option is E. cation exchange chromatography at pH 9. The cation exchange at pH9 will be able to separate histdine and arginine because the pKR of histdine and arginine are on the two sides of pH 9. When pH = pKR, the concentration of HA is equal to the concentration of A. This will help to separate histdine and arginine.

ID: 187064 • Letter: 3

Question

37. The pK1, pK2, and pKR of the amino acid histdine are 1.8, 9.3, and 6.0, respectively. The pK1, pK2, and pKR of the amino acid arginine are 1.8, 9.0, and 12.5, respectively. You have amixture of histidine and arginine, how would you try to separate these two amino acids? MOST IMPORTANLY IS WHYYYYYYYY??????

A) anion exchange chromatography at pH 2

B) anion exchange chromatography at pH 4

C) cation exchange chromatography at pH 2

D) cation exchange chromatography at pH 4

E) cation exchange chromatography at pH 9

Explanation / Answer

The correct option is E. cation exchange chromatography at pH 9.

The cation exchange at pH9 will be able to separate histdine and arginine because the pKR of histdine and arginine are on the two sides of pH 9. When pH = pKR, the concentration of HA is equal to the concentration of A. This will help to separate histdine and arginine.

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37. The pK1, pK2, and pKR of the amino acid histdine are 1.8, 9.3, and 6.0, resp

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