The purity of DNA extractions is measured by calculating the ratio between the a
ID: 186619 • Letter: T
Question
The purity of DNA extractions is measured by calculating the ratio between the absorbance peak of DNA (A260) and the absorbance peaks of common contaminants such as proteins which have peak absorbance at 280 nm (A280), or chaotropic salts which absorb stronl at 230 nm (A230). For DNA, an A260/A280 ratio of ~1.8 and/or an A260/A230 ratio of 1.8-2.2 is generally accepted as "pure". What are the A280 and A230 OD values for your DNA extraction? Based on these values, how pure is your DNA extraction (show your work)? If it isn't pure, what do the ratios indicate are contaminating your extraction? (4 points) 8.Explanation / Answer
if purity of your DNA the ration of A260/A280 is ~1.8 or nearer, than it is a pure DNA but if the ration is 2.0 or higher, it may be RNA contaminant and ration lower than 1.8 the extracted DNA may have impurities of protein, phenol and other organic compound. if the ration of A260/A30 is too lower than the extracted DNA is contaminated with chaotropic salt.
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