Answer these next questions from this graph and the other questions if you can f

Question

Answer these next questions from this graph and the other questions if you can from previous bio knowledge thanks in advance

What does a small Rf value tell you about the charecteristics of the moving molecules?

Which are more solubule in chromatography solvent: Xanophylls or chlorophyll a?

Would you expect the Rf value of a pigment to change if we altered the composistion of the solvent? Why or why not?

if yellow xanophylls were present in the extract, why did the extract appear green?

Is it possible to have an Rf value greater than 1? Why or why not?

Pigment Band Distance from Orgin Rf Value Carotene 14.2 0.99 Xanophyll 0.01 0.0067 chlorophyll a 2 0.13 chlorophyll b 1 0.067

Explanation / Answer

Rfvalue, or retention factor, is the ratio of distance travelled by the solute and the distance travelled by the solvent. Rf value depends on the nature of solvent as well as nature of solute.

If the Rfvalue is more it implies that solute is dissolving in the solvent and vice-versa.

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