2. Vectors used in gene cloning usually are 3. Digestion of DNA by restriction e
ID: 166591 • Letter: 2
Question
2. Vectors used in gene cloning usually are 3. Digestion of DNA by restriction enzymes 4. The enzyme thet acts as molecular glue to join DNA fragments toghter is 5. Agarose gel electrophoresis separates nucleic acid fragments according to 6. In agarose gel electrophoresis the DNA molecule moves towards 7. An ideal vector 8. The important components of s DNA cloning reaction are each of the following EXCEPT 9. A DNA sequencing reaction mixture contains limiting amounts of QUESTION 1 Restriction endonucleases A. recognize and cut specific sequences in the DNA molecule B. synthesize complementary DNA from an RNA molecule C. join the insert DNA with the vector DNA molecule D. are used to run as standard in DNA gel electrophoresis QUESTION 2 Vectors used in gene cloning usually are A. bacteria B. plasmids C. nucleotides D. RNAExplanation / Answer
1. The answer is ‘A’
These enzymes generally found in bacteria and act in defense mechanism against invading viruses. The enzymes selectively cut up foreign DNA, the process called restriction. At the same time the host DNA is protected by a modification enzyme – methyltransferase that modifies the prokaryotic DNA and blocks cleavage.
2. The answer is ‘B’
Plasmids are double-standard, circular DNA sequences, that autonomously replicates inside the bacterial cell.
3. The answer is ‘C’
Restriction enzyme cuts DNA segments within a specific nucleotide sequence and generally palindromic. Restriction digestion is most commonly used in molecular cloning process.
4. The answer is ‘B’
If two DNA molecules have the matching ends, they can be joined by the enzyme DNA Ligase. DNA ligase seals the gap between the molecules forming a single piece of DNA.
5. The answer is ‘D’
Agarose gel electrophoresis is the most effectively used in separating DNA fragments of varying sizes ranging from 100 bp to 25 kb
6. The answer is ‘A’
The negative charge of DNA phosphate backbone moves towards the positively charged anode during agarose gel electrophoresis. The gel matrix is therefore responsible for the separation of DNA by size during electrophoresis.
7. The answer is ‘D’
DNA molecule has the ability to replicate inside the host cell to which the desired gene integrated for cloning is called vector.
8. The answer is ‘D’
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