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2. Use this representation to answer the following questions DNA template strand

ID: 211219 • Letter: 2

Question

2. Use this representation to answer the following questions DNA template strand DNA complementary strand 3" 5 5' 3' If the above segment of DNA is TRANSCRIBED, what enzyme will perform this task? (05ps) (B) In what direction will the to left? (0.5 pts.) enzyme from (A) move along the above DNA template strand: left to right or right move al (C) Suppose the figure from above shows a segment of a typical eukaryotic protein-coding gene. drawing of the RNA that would be produced from transcription of this gene, 1 ndicate on this drawing (adding ents if necessary) where you find the following: stop codon, start codon, S'UTR, 3'UTR (0.5 pts.). Exon 1 Intron 1 Exon 2 Intron 2 Exon 3 100 bp 75 bp 50 bp 70 bp 25 From the RNA transcript from above, draw the fully processed, mature mRNA, indicating all the modifications that must be completed before TRANSLATION can begin, and being sure to indicate the 5 and 3' ends and indicating the complete size, in base pairs of the mature mRNA (assume on your calculations a poly-A tail of 200 A's. (0.5 pts.) (D) (E) What would be the maximum number of amino acids that would be found in the polypeptide produced from translation of this mRNA? (0.5 pts.)

Explanation / Answer

2.
DNA template strand- 5'ATGATCGACTAA 3'
DNA complementary strand would be- 3'TACTAGCTGATT 5'

A.
In order to transcribe a gene, an enzyme known as DNA polymerase is needed.

B.
If DNA polymerase move along the DNA template strand, it would move in right to left direction
as DNA is always synthesized in 5' to 3' direction.

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