Hi, so I answered these questions to the best of my ability. Some are a complete

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Hi, so I answered these questions to the best of my ability. Some are a complete guess. I would EXTREMELY appreciate it if someone could spare some time and explain these and offer more elaboration or even if my answers are completely wrong, new suggestions. I would really appreciate one of the experts who are familiar with all these questions and can help me with them all. I will be forever grateful! THANK YOU SO MUCH --

08. In studying two newly discovered, similar species of birds, l discover the following: In vitro, sperm of one species will only interact with eggs of the same species The same set of proteins are found in the plasma membrane of the sperm ofboth species Similarly, the same set of proteins are found in the plasma membrane of the eggs of both species The amino acid sequences of the proteins found in the sperm plasma membrane are identical in both species Similarly, the amino acid sequences of the proteins found in the egg plasma membrane are identical in both species Based on all these data, what is the BEST hypothesis for how the sperm-egg interactions are made species- Specific? Hypothesis: each species has a unique amino acid sequences 09. I have a rats with either wild type or mutant Pyruvate dehydrogenase (PDH). Imeasure the enzyme kinetics of Liver PDH from these animals in conditions of low or high blood glucose. My data is shown (assume that the mutant responds identically to wild type with all allosteric regulators). Based on all this, what is the MOST LIKELY problem with the mutant PDH? Explain your answer Affinity for mutant is increased while wild form is decreased Not sure what the problem is. High blood glucose Low blood glucose 10. l take equal numbers of liver cells and incubate them with radiolabeled Glucose either in buffer only, or buffer Glucagon. I measure the level of radiolabeled pyruvate in the cells after 30 mins. Cells that are incubated with buffer+Glucagon convert ~5% of the Glucose to pyruvate. How much of the Glucose would have been converted to pyruvate in the cells incubated with buffer only? Explain your answer Less than 5% because glucagon wouldn't be inhibited by glucagon 11. linject equal amounts of radiolabeled glucose into the thigh muscles of sleeping mice, awake mice, or mice running on a treadmill. Two hours later, l measure the amount of radiolabeled glucose present in the blood. My data is shown in the graph. What is the BEST explanation for my results? Running muscles produced lactate that converted to glucose in the liver and released back in the blood or Awake Running Sleeping A. running mice made more glucose for energy B. The running mouse secreated the highest amount of glucagon 12. l isolate liver cells from wildtype and mutant mice under conditions of low blood glucose. The Western blot (using an antibody against PFK2) for these cells is shown. There are no relevant differences in the SDS-PAGE of the PFK2-PO wildtype and mutant. Based on these data, predict MOST LIKELY the PFK2 physiological difference/s of the mutant animals' response to low and high blood sugar (compared to the responses seen in the wildtype). Explain Mutant w respond poorly to changes in blood sugar because it doesn't have PFK2 Rate of glycolysis will be higher in the mutant strain

Explanation / Answer

Answer 8

Interaction between sperm and egg is species-specific; and this interaction does not depend on proteins produced by either sperm or egg. This is understood because not even proteins, but, their amino acid sequences are also same in eggs/sperms of both species.

So, this interaction depends on non-protein sources and certain signals and receptors which reside in sperm and egg respectively.

So, the hypothesis should be:

“Each species has its unique signaling mechanism, by which it interacts with other species of its own kind.”

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