In order to bend membranes N-BAR needs to first bind membranes. To assess BAR bi
ID: 165717 • Letter: I
Question
In order to bend membranes N-BAR needs to first bind membranes. To assess BAR binding to a lipid bilayer adsorbed to mica, a recombinant fusion protein of N-BAR linked to five I-domains with different force phenotypes (same pulling speed) was constructed and the concentration dependence of the Ni-tip tethered N-BAR was measured. Conditions were: pH 8, ionic strength 0.15M and 20 degree C. In each of the traces, describe where failure occurs, the contour L and force of recBAR and force. What roles do the Ig domains play? Why does concentration of free N-Bar influence binding strength of tethered N-Bar. Describe inExplanation / Answer
The given BAR domains are free with N-terminal. Such terminals help protein to maintain banana or L shape. N-BARs bind to curved membranes and help in its regulation for the formation of vesicles. The shape of N-BAR needs force to bind properly with the membrane. Forces of recBARs are responsible for the potential regulation of membrane curvature. More force will help to tightly bind with membrane and thus increase the regulation. Immunoglobulin g is responsible for the signaling pr
The given BAR domains are free with N-terminal. Such terminals help protein to maintain banana or L shape. N-BARs bind to curved membranes and help in its regulation for the formation of vesicles. The shape of N-BAR needs force to bind properly with the membrane. Forces of recBARs are responsible for the potential regulation of membrane curvature. More force will help to tightly bind with membrane and thus increase the regulation. Immunoglobulin g is responsible for the signaling process of curvature maintenance.
Free N-BARs provide extra force to contact with membrane. That increases the relative binding of Ni-tethered N-BAR in compare with fewer amounts of free N-BARs.
To calculate membrane binding energy we would use following formula:
[Force x extension]/ total N-BAR concentration [unit will be ncal/umol]
ocess of curvature maintenance.
Free N-BARs provide extra force to contact with membrane. That increases the relative binding of Ni-tethered N-BAR in compare with fewer amounts of free N-BARs.
To calculate membrane binding energy we would use following formula:
[Force x extension]/ total N-BAR concentration [unit will be ncal/umol]
Related Questions
drjack9650@gmail.com
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.