1.) Briefly state the function of the following repair system in E. coli and ind

Question

1.) Briefly state the function of the following repair system in E. coli and indicate which repair system is associated with the following, genes, enzymes or characteristics.

1. Light-dependent repair ->

2. Base Excision repair ->

3. Nucelotide Excision repair ->

4. Mismatch repair ->

5. Post-replication repair ->

6. Error-prone repair system (SOS) ->

MutH

RecA

Photolyase

uvrB

phr gene

glycosylases

homologous recombination

Lex A

hemimethylated GATC

AP site                               

DNA polymerase III              

recBCD

2.) A mutation of a DNA strand in which a purine replaces a purine and a pyrimidine replaces a pyrimidine is called a ___________________.

ANS:

A mutation of a DNA strand in which a purine replaces a pyrimidine and vice versa is called a _______________________.

ANS:

Indicate if the following is a transition or transversion.

A à G                       

A à C           

T à C            

T à A            

3.) State the type of mutation that would be observed with the following mutagens and indicate the base changes that would result:

5-bromouracil             

Nitrous Acid              

Hydroxylamine          

Alkylating Agents      

Acridine Dyes            

Explanation / Answer

2). A & G---transition

A & C ---- transversion

T & C --- transition

T & A ---- TRasversion

3).5- Bromo uracil---- Adenine to Guanine

Hydroxyleamine ----- Guanine to Cytosine

Alkylating agents ----- Guanine to thymine

Acridine dyes --- Frame shift mutation......

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