I know the answer to 2a, so PLEASE ANSWER 2B You have also performed a pyroseque

Question

I know the answer to 2a, so PLEASE ANSWER 2B

You have also performed a pyrosequencing reaction on the DNA. The order of addition of deoxynucleotide triphosphates is (1) dATP, (2) dCTP. (3) dGTP. and (4) dTTP, and this pattern is repeated. The template DNA sequence is 5'-ATTGTCGGTTGATTA-3' a. You forgot to add sulfurylase in the first reaction (following the first dATP addition), but remembered to add it for all subsequent reactions. What will be the consequence of this mistake? Explain. You forgot to add apyrase following the second round of dTTP addition (i.e, (the second time dTTP was added). What will be the consequence of this mistake? Explain.

Explanation / Answer

apyrase removes unincorporated nucleotides and ATP between additions of different nucleotide only. unwanted nucleotides will only affect the reaction cycles and thus, interferes with the results.

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