5. All of your YPD patches will be replica printed onto a MATa lawn and onto a M

Question

5. All of your YPD patches will be replica printed onto a MATa lawn and onto a MATalpha lawn. Each of those replicas will be incubated for 48 hours and then printed onto SD. You will therefore have two SD plates, one originating from the MATa tester replica and one originating from the MATalpha tester replica.

a. Should any of the specific patches that arise on the SD plate originating from the MATa tester replica also arise on the SD plate originating from the MATalpha tester replica? (i think the answer is yes)

b. If you have a diploid patch on your YPD patch plate, will that colony result in growth on the SD plate originating from the MATa tester replica?

c. If you have a diploid patch on your YPD patch plate, will that colony result in growth on the SD plate originating from the MATalpha tester replica?

Explanation / Answer

From the Yeast Postato Dextrose (YPD) media plate we took yeast cells to grow them and then to transfer them on a MAT lawn. here we have two types of MAT lawn, one is MATa which we know as types having a deficiency in an enzyme to syhthesize tryptophan (trp) while if we see the MATalpha types have a deficiency in adenine (sde) synthesizing enzyme. This parimart idea of ours will be helpful answering and understanding the rest 3 questions.

a. We must know that SD plate is a minimal media plate, and it lacks all the essential amioacids, thus if we want to see any kinds of growth of yeast cells, it must be having the original strength with the amino acids production.

b. Here we have the idea that SD plate conatins minimal media, so no amio acids are supplied externally. Now we have MATa lawn plate, which lacks ade, so that will not help the diploid cells growing in minimal media.

c. The concept of complementation helps us understnading this question. To easily distinguish the haploid and diploid strains of Yeast cells. As we have seen SD plate contains minimal media, where additional nutrients are not supplied, so cells cannot make the trp amino acids, therefore will not grow xoming from MATalpha plate.

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