Figure 1: SDS PAGE Gel of Murine Fibroblast Cell Lysate samples. Lane 1, 5 µL pr

Question

Figure 1: SDS PAGE Gel of Murine Fibroblast Cell Lysate samples. Lane 1, 5 µL protein marker added. Lane 2, sample 3 containing 5 µL of a 16 µL of cell lysate solution. Lane 3, containing of 5 µL of a 9.6 µL cell lysate and 6.4 µL distilled water dilution solution. Lane 5, containing 5 µL of an 8 µL Bovine Serum and 32 µL distilled water solution. Lane 6, containing 5 µL of a 4 µL Bovine Serum and 16 µL of distilled water solution. Lane 7, containing 5 µL of a 2 µL Bovine Serum and 8 µL of distilled water solution. Lane 8, containing 5 µL of an 8 µL Horse Serum and 32 µL distilled water solution. Lane 9, containing 5 µL of a 4 µL Horse Serum and 16 µL of distilled water solution. Lane 10, containing 5 µL of a 2 µL Horse Serum and 8 µL of distilled water solution

Read from left to right: Lane 10, Lane 9, Lane 8, Lane 7, Lane 6, Lane 5, Lane 4, Lane 3, Lane 2. Lane 1

1. Using the provided protein marker standard, estimate the molecular weights of each band on the gel and identify the protein that may be present

Explanation / Answer

The molecular weights of the bands in the gel are mentioned in the colored lane. It started from 10kbp to 250kbp.

The corresponding bands of the sample lane to that of the marker lane show that the sample DNA formed distinct bands at 10kbp, 15kbp, 25kbp, 37kbp, 100kbp and then at 250kbp.

All other bands are almost negligible. It indicates that 16ul of cell lysate consisted of

10+15+25+37+250+100 = 437kb

9.6ul of cell lysate consisted of (9.6*437)/16 = 262kb

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