Figure 1: SDS-PAGE Gel of Murine Fibroblast Cell Lysate, Bovine Serum and Horse

Question

Figure 1: SDS-PAGE Gel of Murine Fibroblast Cell Lysate, Bovine Serum and Horse Serum samples. Lane 1, 5 µL protein marker added. Lane 2, sample 3 containing 5 µL of a 16 µL of cell lysate solution. Lane 3, containing of 5 µL of a 9.6 µL cell lysate and 6.4 µL distilled water dilution solution. Lane 5, containing 5 µL of an 8 µL Bovine Serum and 32 µL distilled water solution. Lane 6, containing 5 µL of a 4 µL Bovine Serum and 16 µL of distilled water solution. Lane 7, containing 5 µL of a 2 µL Bovine Serum and 8 µL of distilled water solution. Lane 8, containing 5 µL of an 8 µL Horse Serum and 32 µL distilled water solution. Lane 9, containing 5 µL of a 4 µL Horse Serum and 16 µL of distilled water solution. Lane 10, containing 5 µL of a 2 µL Horse Serum and 8 µL of distilled water solution.

Observe: From left to right: Lane 10, Lane 9, Lane 8, Lane 7, Lane 6, Lane 5, Lane 4, Lane 3, Lane 2. Lane 1

Discuss TWO protein bands from EACH SERUM sample and BOTH bands for lanes 1-2 in terms of:

1. Size: Estimate molecular masses and relative abundance of unknown polypeptides in a complex mixture
2. Patterns of bands that suggest presence of proteins
3. Compare and contrast one band to another
4. Guess what proteins are in the serum shown in the gel result (research using Biorad, Precision Plus protein or dual color standards)
5. Discuss and critique gel quality: Overloading, under-loading, distortion of lanes, non-reproducible patterns, smears, streaks, etc.

Explanation / Answer

1. Size: Estimate molecular masses and relative abundance of unknown polypeptides in a complex mixture

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