1. Which of the following experimental approaches can be used to identify bacter
ID: 151913 • Letter: 1
Question
1. Which of the following experimental approaches can be used to identify bacteria that have the ability to survive in the presence of chemicals that cause oxidative stress?
a) Gram stain
b) Malachite Green Stain
c) Oxidase Test
d) Catalase Test
e) Oxidative Test
2. Which of the following would be an additional biochemical assay that can be used to characterize bacteria?
a) Gram stain
b) PCR
c) 16S rRNA gene sequencing
d) Streak plate test
3. Which of the following best explains why ribosomal RNA gene sequences can be used for the identification of bacterial species?
a) Distantly related bacterial species have 16S rRNA genes that are highly similar to each other
b) Distantly related bacterial species have 18S rRNA genes that are highly similar to each other
c) Closely related bacterial species have 16S rRNA genes that are highly similar to each other
d) Closely related bacterial species have 18S rRNA genes that are highly similar to each other
e) Closely related bacterial species have 16S rRNA genes that are highly dissimilar from each other
e) Urease test
4. What is the function of the protein encoded by Bacterium A GeneA1 (Escherichia coli E2863 DNA, complete genome):
a)Shiga Toxin
b)Resistance to beta lactam antibiotics
c)Synthesis of peptidoglycan
d)Catalase enzyme
e)Oxidase enzyme
5.What is the function of the protein encoded by Bacterium B GeneB1 ( Staphylococcus aureus strain QD-CD9 chromosome, complete genome):
a) Shiga Toxin
b) Resistance to beta lactam antibiotics
c) Synthesis of peptidoglycan
d) Catalase enzyme
e) Oxidase enzyme
Explanation / Answer
1 Answer is c) Oxidase test.
This test is used to identify microorganisms containing the enzyme cytochrome oxidase (important in the electron transport chain). It is commonly used to distinguish between oxidase negative and oxidase positive.
2. Answer is c) 16S rRNA gene sequencing
The use of 16S rRNA gene sequences to study bacterial phylogeny and taxonomy.
3. Answer is a) distantly related bacterial species have 16S rRNA genes that are highly similar to each other.
It is suggested that 16S rRNAgene can be used as a reliable molecular clock because 16S rRNA sequences from distantly related bacterial lineages are shown to have similar functionalities.
4. Answer is c) synthesis of peptidoglycan
5. Answer is b) Resistance to beta lactam antibiotics
Related Questions
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.