Please read and answer the following question. Please try not to write in cursiv
ID: 150646 • Letter: P
Question
Please read and answer the following question. Please try not to write in cursive. Ignore question 4 and 5 at the top.
A. How might you select for the hybrid you want?
b. What is the difference between intraspecific protoplast fusion and interspecific protoplast fusion?
4. Apart from your Gene of Interest, what other genes/gene sections might have to be added? 5. How will you test for a successful cell modification? Using Protoplast Fusion to Create "Spiderman" Protoplast Cultures fuse together two cells that have no cell wall. Scientists use various mechanical (micropipette), electrical (electrofusion) or chemical (polyethylene glycol) means to encourage the cells to form a hybrid. Because these methods are random and uncontrolled, the protoplasts rarely form a true hybrid. However, if (when) a hybrid is formed, it may regenerate a cell wall, grow, and divide. This hybrid culture can expand using special media. This technique is used mostly for plants, fungi (yeast) and plant:fungi hybrids. It has been used to improve the production of cephalosporin C and increase plant resistance to certain viruses.Explanation / Answer
Answer1- Drug sensitivity is useful for the hybrids of two plant speices, if one of them is sensitive to a drug. For example, Protoplasts of Petunia hybrid can form macroscopic callus on MS medium, but are sensitive to actinomycin D. Petunia parodii protoplasts form small colonies, but are resistant to actinomycin D.
Answer 2- Intraspecific protoplast fusion- Hybrids are produced between different varieties of the same species. e.g.- between non- flowering potato plants and flowering potato plants.
Interspecific protoplast fusion- Hybrids are produced between two different species. e.g.- between Triticum and rye Secale to produce Triticale.
Related Questions
drjack9650@gmail.com
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.