Please read and answer the following question. Please try not to write in cursiv

Question

Please read and answer the following question. Please try not to write in cursive.

A. Which of these sections must be ‘deleted’ before using this Ti Plasmid to genetically engineer plants?

B. How will you test for a successful cell modification?

Using the Ti Plasmid to Create GMO Plants The Ti Plasmid, gene guns, electroporation and microinjection can be used to create genetically modified crops (GMCs) which are healthier, resistant to insects, herbicide tolerant or produce more fruit. Some examples are: FlavrSavr tomato (1994) longer shelf life, slower ripening Tobacco plant resistant to herbicide bromoxynil (1994) BT Potato (1995) produces pesticide Cotton plant (1995) resistant to bromoxynil Soybeans (1995) resistant to glyphosphate Golden Rice (2000) vitamin-A enriched rice Agrobacterium tumefaciens use their Ti Plasmids to insert genes into plant cells in order to create a suitable environment for them. Genes on the Ti Plasmid create a 'crown gall' in the plant. The bacteria bind to the plant cell and inserts the T-DNA into a random site of the plant genome.

Explanation / Answer

(PART A) = The section between Left and right order which include Cytokin, Auxin and Opines must be deleted before using Ti Plasmid to genetically engineer plant.

The presence of genes encoding opines and Growth regulators is not favorable for the production of transgenic plants – the expression of these genes affects and can prevent the regeneration of the transformed plants.

(PART B) = The new genetic material can be inserted randomly within the host genome or targeted to a specific location. uses homologous recombination technique to make desired changes to a specific endogenous gene.

THANK YOU.

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