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.Deterweining the lameter of the Fleld of ew t. Rotate the low-power objective i

ID: 150527 • Letter: #

Question

.Deterweining the lameter of the Fleld of ew t. Rotate the low-power objective into the light ath What is the total magnification, 40 2. Place a transparent 15-cm nuler on the stage 3. What is the diameter of the field of view? 4 Repeat step 3 with the medium-power objective in the light path. The total magrut The diameter of the field of view ismm ification is S. ise che following formula to estimate the diameter of the field of view when the high-dry objective is in thee light path total magnification mm counted using low-power x with that total magnification of high-dry objective Once you've cakculated this value, convert it to micrometers:um 6. Complete Table 4-5 TABLE 4.5 Diameter of Field of View Magnitying Power Diameter of Fiold of! View Objective High-dry 7. You will use this information to estimate size by observing the percentage of the diameter of the field of view taken up by the specimen or part of the specimen. Using the medium-power objective, estimate the etter e is percent × diameter of field of view (mm) tage of the diameter of the field of view covered 100% 8. If your microscope is equipped with an ocular micrometer, your instructor may provide directions on how to accurately measure specimen details. 4.2 How to Make a Wet Mount (About 10 min.) In the mid-sevententh century, Robert Hooke used a microscope to discover tiny, empty compartments in thin shavings of cork. He named them cells Repeating this historic observation is a good way to learn how to prepare a wet mount MATERIALS Per student

Explanation / Answer

1. Total magnification = eyepiece × objective

3. Diameter of field of view under low power = 4mm

4. Total magnification in medium power = 10 × 10 = 100

5. Feid of view when high dry objective is used = (total magnification in low power × field of view under low power) ÷ total magnification under high dry

6. Low power

Medium power

High dry

Oil immersion

7. It will depend upon the size of letter that you will draw

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