The following is a segment of DNA containing a palindrome. CTACGAATTCGTCT GATGCT

Question

The following is a segment of DNA containing a palindrome. CTACGAATTCGTCT GATGCTTAAGCAGA A restriction enzyme that recognizes this sequence produces breaks four nucleotides apart from the axis of symmetry in each strand. The break leaves a 5' overhang. Show the fragments that can be generated with the complementary single-stranded projections. a. b. A restriction enzyme that recognized this produces breaks two nucleotides apart from the axis of symmetry in each strand. The break leaves a 3' overhang. Show the fragments that can be generated with the complementary single-stranded projections.

Explanation / Answer

C T A C G A A T T C G T C T

G A T G C T T A A G C A G A

a.)

EcorI digestion of restriction site: GAATTC

5'- C T A C G - 3'                           5'- A A T T C G T C T - 3'

3'- G A T G C T T A A - 5'                          3' - G C A G A - 5'

b.)

There are no restriction enzyme which is capable of generating 3' overhang from the above mentioned DNA sequence. Only 5' overhang could be generated, not the 3' overhang.

Bioinformatics tools such as restrictionmapper, etc. finds restriction site in a DNA sequence. These tools could not detect 3' overhang producing restriction enzyme for the above sequence, but it could detect 5' overhang producing restriction enzyme for the above sequence.

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