Two codons (CAG and CAA) are used for the amino acid glutamine. (10 points in to

Question

Two codons (CAG and CAA) are used for the amino acid glutamine. (10 points in total) 8. What is the minimal number of tRNA-Gln needed to read these codons? List the anticodon(s) expected for each predicted tRNA-Gin. (3 points) a. Ankcodons O for cA6:GUC An amber suppressor (sup-2) is due to a mutation in a glutamine tRNA gene. What anticodon would you expect this suppressor tRNA to have? (2 points) b. AU C c. An ochre suppressor CupE)is also due to a mutation in a glutamine tRNA gene. What anticodon would you expect this suppressor tRNA to have? (2 points) cUA d. A double-mutant carrying both suppressor mutations (sup-2 and supE) can be made. The double-mutant suppresses both the amber and ochre mutations and

Explanation / Answer

As the double mutation supresses the amber and ochre mutation, the minimal number of glutamine trna genes are CAG and CAA.

The anti-codons are GUC and GUU

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